Dangers of Control from the Cryogenic Liquid Nitrogen Storage Container and Cryopreservation of Embryo and Sperm of IVF laboratory

Moderator ; MD / Dr Ritu S,Santwani
– Obst & Gynaec.
FICOG – FIAOG AMRCOG –ART-Singapore IVF specialist Director Pune Test Tube Baby center & shyam well Woman Clinic – India.

Moderator : Ms. Azhar Ismail
Department of IVF Lab.
Clinical Embryologist Experience more than 20 years
Worked at Michigan IVF – Lebanon.

Speaker / Dr. Mustafa ZAKARIA Msc
MSc Reproductive Biology, Deputy Executive Director and Administrative Coordinator of the Society for Art Irifiv Scientific Research Group (AISRG) and Consultant in IRIFIV Fertility Center , IVF laboratoy, Casablanca, Morocco

Description ; what are the risks associated with Embryo freezing?
1-Risk Of Contamination ;Since viruses and bacteria can also survive at cryogenic temperatures it needs special attention.
The risk of cross-contamination, i.e., transfer of bacteria or other microorganisms from liquid nitrogen to stored samples, is should be taken care.
2- Risk Of Reduced Or Lost Viability In Embryo Freezing: In Embryo freezing Long-term storage does not impact viability/developmental potential of slow-frozen embryos. Cryostorage appears to be safe for slow-frozen oocytes as well.
3- Risks Inherent In Shipment And Handling;At times, frozen eggs or embryos need transportation for further use according to patient convenience. The shipping procedure carries risk of vessels being exposed to elevated ambient temperature and air pressure, vibration/other physical shock, and horizontal storage.
4- Risk Of Specimen Loss In Frozen Embryo:In contrast to the risk of infection or time-related decrease in viability, under current practices the risk of loss of cryopreserved gametes and embryos due to human error or equipment failure is relatively high.
It is the responsibility of the IVF laboratory to take proper care of the procedure in vital procedures like cryo-freezing.
5- Embryo freezing is a type of fertility preservation
:Embryo freezing may be useful for women with cancer who want to have children after having radiation therapy, chemotherapy, or certain types of surgery, which can cause infertility. Also, called embryo banking and embryo cryopreservation it is widely used in IVF treatment.

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