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Chaymaa Kabir-Idrissi1, Achraf Zakaria3, Mustafa Zakaria4, Sabri Yassir1, AbdelghaniMrini1, Bouchra El Khalfi2, Abdelaziz Soukri2, Modou Mamoune Mbaye*2,3,4 and NoureddineLouanjli 2,3

Just like in women, the causes of infertility in men are many and varied. To remedy
this, several couples resort to medically assisted procreation (PAM). However, the latter
comes up against the problem of the sperm quality of the donor. For this, subnormal
semen samples are treated with specific chemical agents before artificial insemination
to improve the fertilizing power of the sperm. Because sperm health plays a decisive
role in male fertility, survival is correlated with the fertilizing power of sperm. It is in
this perspective that our study is part of our study consisting in following the survival
of the sperm after improvement and incubation at 25 °C versus 36 °C in order to find
the optimal temperature of incubation for an apparent increase. Poor results from
in vitro fertilization (-20%). The results of the effect of incubating samples at 25 °C
and 36 °C on the mobility and vitality of human spermatozoa which were followed in
different incubation times (1,2, 3 and 4h) showed that ‘after 1 hour of incubation the
mobility and vitality at 25 °C dropped considerably compared to that of the sample at
36°C. After 2 hours of incubation time, we note that the two samples drop drastically
but still with the samples at 36°C which have kept the best mobility and vitality. This
same phenomenon was also noted at 2 and 3 hours of incubation at 25 and 36 °C.
It should be remembered that the most favorable temperature was 36°C because the
highest fertilization rate was noted at this temperature

Article History
Received 2 June 2021
Revised 20 July 2021
Accepted 22 July 2021
Available Online 23 July 2021

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